Yap and Taz function as the osteochondrogenic determinant in neural crest cells

• DOI: 10.1096/fasebj.2021.35.s1.04713
• Publication Date: 2021-06-11T01:05:50Z
• Authors: Xiaolei Zhao; Li Tang; Bao Nguyen; Tram Le; Wen Chen; Mingjie Zheng; Hiroyuki Yamaguchi; Idaliz Martinez‐T...; James Martin; Jianxin Wang; Min Li; Brendan Lee; Yoshihiro Komatsu; Jun Wang
• Keywords: Chondrogenesis; RUNX2; Cell fate determination; Hippo signaling pathway

The neural crest cells (NCCs) are multipotent stem making pivotal contributions to many tissues and organs during embryonic development. NCCs have remarkable ability differentiate into various cell lineages including osteoblasts chondrocytes, yet it is largely unknown how make fate decisions specific lineages. Here we found Yap/Taz required for NCC-derived differentiation notably, deficiency caused switch from osteogenic chondrogenic in vitro. In vivo deletion cranial bone defects ectopic cartilage formation mouse. We performed a combined analysis of data sets RNA-seq Reverse Phase Protein Array (RPPA) control deficient samples, ATAC-seq (assay transposase-accessible chromatin with high-throughput sequencing) human mouse NCCs, CUT&RUN (Cleavage Under Targets & Release Using Nuclease)-seq using Yap antibody, as well immunoprecipitation data. directly regulate key genes osteogenesis chondrogenesis Sox9 Runx2 that the fates osteoblast chondrocyte lineages, other such Sox5, Sox6, Osx. This regulation partially through interacts β-catenin coordinately expression at genome-wide. Together, demonstrate play roles derived osteochondrogenic decision, which functions interaction Wnt-β-catenin pathway.