Isolation and Characterization of Antimicrobials from Icelandic Aquatic Bacteria

• DOI: 10.1096/fasebj.2022.36.s1.0r442
• Publication Date: 2022-05-03T09:09:46Z
• Authors: Irene Corona‐Avila; Jeongho Lee; Antonio Hernandez; Nyssa Krull; Mario Augustinovic; Derick Jones; Tatyana Ndekwe; Brian T. Murphy
• Keywords: Bacterial growth

Since the Golden Age of natural product (NP) antibiotic discovery in mid-1950s, NP has declined despite methodological advances. As a result, antibacterial is being outpaced by resistance. One significant challenge rediscovery compounds previously isolated from bacteria. Generating diverse microbial library can help overcome compounds, but major challenges to this approach include inefficiency commonly used methods create high-throughput libraries bacteria and difficulty reducing redundancy within these libraries. To combat issues, Murphy lab works with an innovative using IDBac, efficient, rapid process developed reduce taxonomic chemical In current study, we performed liquid chromatographic separations Icelandic bacterial isolates K391, K765, K802. We then investigated each isolate's ability against pathogens through growth inhibition assays. Bacterial isolate K391 displayed weak activity fractions 3-5, 14-15, 25-26 showed presence small molecule, which may hold potential as via MS/MS peaks 14-15. are further investigating GNPS analysis running K765 K802 normal phase.