High‐fat/high‐carbohydrate diet increases glycogen accumulation in lung tissue in vivo

• DOI: 10.1096/fasebj.2022.36.s1.r2115
• Publication Date: 2022-05-03T09:35:39Z
• Authors: Alexis N. James; Lindsey R. Conroy; Tara R. Hawkinson; Josephine E. Chang; Elena C. Manauis; Jessica F. Lamb; Michael D. Buoncr...; Lyndsay E. A. Young; Christine Filmore...; Ramon C. Sun
• Keywords: Tumor progression; Corn oil; Carbohydrate Metabolism

Lung adenocarcinoma (LUAD) is the major histological subgroup of non-small cell lung cancer (NSCLC) with an extremely high mortality rate even when diagnosed at early stage. LUAD makes up around 40% cases and can quickly metastasize to other areas body. Glycogen short-term storage carbohydrates be easily mobilized in Recently, our own data suggests glycogen drive progression, however, mechanism which accumulates poorly understood potentially arises from a number genetic environmental factors. Although specific variants driving aberrant accumulation have been identified, several studies shown that caloric excess as well nutrients diet promote tumor growth. Further, patients type II diabetes or BMI significantly poorer survival. Therefore, we hypothesized rich fats mimics Western increases tissue drives progression. To test hypothesis alters metabolism independent drivers formation, administered via oral gavage comprised corn oil fructose syrup (150 μl oil: 25% high-fructose solution) wild-type C57BL/6 mice acute (1-, 3-, 6-hours) chronic (two weeks) time points. At end each point, were sacrificed was harvested. Using combination gas chromatography mass spectrometry (GCMS) matrix-assisted laser desorption ionization imaging, quantified central carbon metabolites content resected these mice. Mice H2O used control. We found for two weeks exhibited elevated levels metabolite pools within glycolysis TCA cycle compared control group. However, revealed no change 6-hours after receiving diet. Overall, highlight link between suggest intake different dietary may play role tumors disease Our results demonstrate fat/high carbohydrate exposure. Moving forward, will examine contribution mouse assess effect increased on growth vivo.