Including rapeseed protein in a high‐fat meal prevents postprandial vascular endothelial dysfunction in rats

• DOI: 10.1096/fasebj.22.1_supplement.312.4
• Publication Date: 2021-06-21T23:40:28Z
• Authors: Joëlle Magné; Jean‐François Huneau; Daniel Tomé; François Mariotti
• Keywords: 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences

High saturated fat/ high sucrose meals induce oxidative stress associated with vascular endothelial dysfunction, an early hallmark in atherogenesis, but the impact of meal protein on vascular homeostasis remains misunderstood. Here, we investigated the potential of rapeseed protein, an emergent cysteine‐rich protein, to modulate the adverse effects of a high‐fat meal (HFM).In a cross‐over design, 12 healthy rats received 3 isoenergetic HFM (saturated fat: 60%; sucrose: 20%; proteins: 20% of total energy) with the protein source being either total milk protein (C), rapeseed protein (R), or milk protein supplemented with cysteine and arginine up to the same level as in R (AA). Endothelium‐related vascular reactivity, measured as acetylcholine‐induced transient fall in blood pressure, and plasma triglycerides, hydroperoxides and cGMP were measured before and 2, 4 and 6 h after meal.While plasma triglycerides rose similarly in all meals, the decrease in endothelial function after C was attenuated after AA and steadily blunted after R. The postmeal decrease in plasma cGMP was lessened 2h after R, as compared to after meal C. Plasma hydroperoxydes increased after the 3 meals, but were lower 6h after meals AA and R than after C.These results show that rapeseed protein specifically suppresses the acute deleterious effects of HFM on endothelial function, and this may be mediated in part by cysteine and arginine.