Kappa opioids promote the proliferation of astrocytes via Gbetagamma and beta‐arrestin 2 dependent ERK/MAPK‐mediated pathways

• DOI: 10.1096/fasebj.22.1_supplement.645.17
• Publication Date: 2021-06-21T23:45:34Z
• Authors: Carmine James Coscia; Gregory P McLennan; Alexi Kiss; Mayumi Miyatake; Kari T Chambers; John J Pozek; Yasmin Mohabbat; Mariana M Belcheva
• Keywords: Immortalised cell line

Seven‐ transmembrane receptors activate ERK/MAPK via G protein dependent and independent mechnanisms. Opioid (ORs) modulate proliferation of immortalized primary astrocytes ERK. We examined κ OR‐induced, ERK‐mediated rat to delineate participation vs. β‐arrestin (β‐arr). opioid agonist, U69,593, activated ERK by a rapid (min) initial stimulation that was sustained over 2 h increased as measured bromodeoxyuridine labeling. MOM‐Sal‐B (a salvinorin derivative), induced activation transiently did not affect suggesting activity is required. In astrocytes, elicited proliferation. Transfection with cDNA Gβγ scavenger reduced activaty U69,593. Treatment cells siRNA indicated U69,593 β‐arr dependent. The same inhibitors decreased in cells. or abolished both opioids. results suggest pathways are required elicit astrocytes. Supported NIH DA 05412