Structural basis for midbody targeting of spastin by the ESCRT‐III protein CHMP1B

• DOI: 10.1096/fasebj.23.1_supplement.864.2
• Publication Date: 2021-06-21T22:41:08Z
• Authors: Dong Yang; Neggy Rismanchi; Benoit Renvoise; jennifer Lippinco...; Craig Blackstone; James Hurley
• Keywords: Midbody; ESCRT

The endosomal sorting complex required for transport (ESCRT) machinery, including ESCRT‐III, localizes to the midbody and participates in membrane‐abscission step of cytokinesis. ESCRT‐III protein chromatin‐modifying 1B (CHMP1B) is recruitment MIT domain‐containing spastin, a microtubule‐severing enzyme, midbody. 2.5‐A structure C‐terminal tail CHMP1B with domain spastin reveals specific, high‐affinity involving noncanonical binding site between first third helices domain. structural interface twice as large that VPS4‐CHMP complex, consistent high affinity interaction. A series unique hydrogen‐bonding interactions close packing small side chains discriminate against other ten human subunits. Point mutants block