Effect of remote ischemic preconditioning on hepatic parenchymal and microvascular damage in obesity

• DOI: 10.1096/fasebj.25.1_supplement.1117.9
• Publication Date: 2021-06-21T16:10:56Z
• Authors: Heather A. O'Leary; Anand Kothur; Sara B. Fournier; Adam G. Goodwill; Jefferson C. Frisbee; Robert W. Brock
• Keywords: Ischemic Preconditioning; Parenchyma; Clamp

Remote ischemic preconditioning (RIPC) is an adaptive response in which tolerance to prolonged periods of ischemia/reperfusion (I/R) induced a target organ by prior shorter I/R distant organ. Although this phenomenon has been established within healthy population, the effects RIPC have yet be defined obese population. The benefits coupled with type protection are particular importance since obesity, and confounding factors associated it, known result repeated events. Thus, we set forth determine effect on direct hepatic injury ob/ob db/db mice. For RIPC, utilized protocol 10-min hindlimb ischemia followed reperfusion three times. Partial (70%) was initiated placing atraumatic clamp across portal triad between quadrate median/lateral lobes. Obese mice had significantly higher levels liver parenchymal damage during compared controls (ob/ob: ~10-fold & db/db: ~4-fold). Sinusoidal endothelial cell (SEC) dysfunction also increased ~2-fold After detrimental were improved at least 60%, both SEC dysfunction. Together, these data suggest that hindlimbs extends significant functional livers those obesity.