Persistence of Lactobacillus Reuteri DSM17938 in the Human Intestinal Tract in Response to Different Storage Conditions

• DOI: 10.1096/fasebj.25.1_supplement.584.15
• Publication Date: 2021-06-07T12:12:19Z
• Authors: Tracey J Smith; Danielle J. Anderson; Anthony Sikes; Lee M. Margolis; Andrew J. Young
• Keywords: Lactobacillus reuteri; Persistence (discontinuity); Volunteer

Probiotic-containing foods are consumed to enhance gastrointestinal (GI) and immune function. How storage of those affects persistence probiotics in the GI tract undefined. We evaluated Lactobacillus reuteri DSM17938 (L. reuteri; BioGaia, Stockholm, Sweden) human after consuming “fresh” (FRESH; n = 9; room temperature ≤ 1 week followed by freezer) or “stored” (STORED; 10; 37°C for ~7 days L. reuteri-containing pudding (108 109 CFU/serving, respectively) 7 days. Fecal samples were collected daily during probiotic supplementation (D1–7) intermittently discontinued (D13–15 D20–22), analyzed reuteri. Results reported 3-day increments (D2–4, D5–7, D13–15, D20–22). All volunteers FRESH, volunteer STORED, had detectable (D1–7). count rose FRESH ([mean ± SD] D2–4: 4 x 104 2 104CFU, p < 0.01; D5–7: 10 9 0.01), but not STORED (D2–4: 3 102 102CFU, P 0.3; 0.3). More weeks ended (4/9 2/9, compared (0/10 0/10, respectively). Colonization is achievable with intake “fresh”, Funded Medical Research Materiel Command.