Effect of resveratrol and caloric restriction on mitochondrial regulation and apoptotic susceptibility in aged rat skeletal muscle

• DOI: 10.1096/fasebj.26.1_supplement.1077.5
• Publication Date: 2021-06-21T15:34:19Z
• Authors: Angelina G Malamo; Jason Silvestre; Mark E Adams; Linda M-D Nguyen; Anna M Joseph; Debapriya Dutta; Jinze Xu; Marvin L Dirain; Danny M Tuckerman; Christiaan Leeuwe...; Peter J Adhihetty
• Keywords: Mitochondrial toxicity; Mitochondrial ROS

Sarcopenia is an age‐related loss in muscle mass partially attributable to mitochondrial‐mediated apoptosis. Caloric restriction (CR) and resveratrol (RSV) treatment rodents induces beneficial mitochondrial alterations which may serve suppress sarcopenia. Doxorubicin (DOX) a chemotherapeutic agent that cell death via mitochondria. We investigated whether RSV (50 mg/kg/day; 6 weeks) and/or CR (20% reduced AL; could 1) induce biogenesis, 2) attenuate apoptotic susceptibility and, 3) reduce DOX‐induced toxicity, aged rodent muscle. Aged F344xBN rats (26 mo) were split into 8 groups (n=4): ad libitum (AL), CR, RSV, RSV+CR injected with DOX (20 mg/kg; IP) or saline prior (24h) sacrifice. Mitochondrial content/regulation (cyto c, COX activity, PGC‐1á, SIRT3) (Bax:Bcl‐2) assessed hindlimb Surprisingly, indices unaffected by CR+RSV, did not affect any group. CR+RSV (50%) the Bax:Bcl2 ratio compared AL while independently showed trends for reductions. enhanced Bax:Bcl‐2 tended this DOX‐induction. Our data indicates muscle, DOX‐treatment, increases provides modest protection without altering content/regulation.