Measurement of microcantilever spring constants for probing receptor‐ligand bonding

• DOI: 10.1096/fasebj.26.1_supplement.578.4
• Publication Date: 2021-06-21T15:50:33Z
• Authors: yang liu; David Tees
• Keywords: Equipartition theorem

The force dependence of receptor–ligand bonding has been extensively studied in biophysics. Microcantilevers are a type spring‐based sensor and their spring constants were characterized using different methods to check that the measurements consistent. made from E‐glass fibers with nominal diameter 4 μm lengths 3–7 mm mounted viewing chambers imaged under microscope 100× oil immersion lens. Multiple sets images for each microcantilever captured at 50–800 frames per second analyzed custom LabVIEW IMAQ image processing program. intensity profile was used find edges (represented by two troughs). equipartition theorem constant values displacements time domain. These found decrease increasing cantilever length as expected elastic theory. Fluctuations also frequency Power Spectral Density fitted Lorentzian. calculated Lorentzian parameters consistent results theorem. This work supported funding Ohio University Biomimetic Nanoscience NanoTechnology Program.