Gravin‐Dependent Multi‐Kinase Signaling Modulates Cardiac Function

• DOI: 10.1096/fasebj.26.1_supplement.666.9
• Publication Date: 2021-06-21T15:39:13Z
• Authors: Ashley Nicole Gui...; Paul Tran; Khadijah Bilal; Cori Wijaya; Andrea Diaz Diaz; Bradley McConnell
• Keywords: Phospholamban; Basal (medicine); Knockout mouse

The purpose of this study was to investigate the effect knockout gravin, an A kinase anchoring protein, on regulation cardiac function by protein (PKA) and C (PKC). We measured PKA activity, PKC activity substrate phosphorylation in wild‐type (WT) gravin (KO) mice stimulated with isoproterenol (ISO; 30 mg/kg/day x 14 days). Basal significantly increased both WT KO treated ISO (WT Vehicle: 0.023±0.0041; ISO: 0.098±0.021; 0.020±0.0021; 0.069±0.0090; A.U.; p=0.0023). However, had a decreased response cAMP‐stimulated compared their respective controls 0.44±0.0094; 0.35±0.027; 0.46±0.011; 0.35±0.026; p=0.0043). untreated animals 0.069±0.012; 0.13±0.022; p=0.041). Additionally, there significant increase phospho‐phospholamban group its control (p=0.0151). Furthermore, phospho‐troponin I (p=0.033). These data indicate that absence has profound action PKC. This research supported NIH R01HL085487.