Role of Voltage‐dependent Kv7 Channels in the Regulation of Coronary Blood Flow

• DOI: 10.1096/fasebj.27.1_supplement.1185.4
• Publication Date: 2021-06-21T15:04:49Z
• Authors: Adam G Goodwill; Eli D Casalini; Meredith K Owen; Abass Conteh; Daniel Sassoon; Kashyap Shatagopam; Gregory M Dick; Johnathan D Tune
• Keywords: Coronary circulation; Coronary vasodilator

Previous studies have demonstrated a key role for voltage‐dependent K (K V ) channels in the regulation of coronary metabolic and ischemic vasodilation. However, channel subtypes responsible control blood flow not been clearly defined. This investigation examined hypothesis that 7 are present vascular smooth muscle regulate perfusion myocardium at rest during pacing‐induced increases metabolism. In anesthetized, open‐chest swine, we found intracoronary administration agonist flupirtine (1 nM‐10 μM) or antagonist linopiridine (1–100 did significantly affect baseline (Δ = −0.002 ± 0.005 vs. −0.010 0.004 ml/min/g, respectively). H 2 O increased ~2‐fold this response was unaffected by linopiridine. Whole‐cell recording isolated cells revealed linopirdine both inhibited current ~25 3% ( P < 0.05). Cardiac pacing (57 + 5 to 102 12 bpm) myocardial oxygen consumption (~35%) (~38%). linopridine (10 alter increase slope relationship between 0.61). These data do support prominent flow. (Support: HL092245)