Mitophagy as a quality control mechanism in Saccharomyces cerevisiae

• DOI: 10.1096/fasebj.27.1_supplement.994.3
• Publication Date: 2021-06-16T10:22:56Z
• Authors: Hagai Abeliovich; Mostafa Zarei; Kristoffer T.G. R...; Richard J. Youle; Joern Dengjel
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences

The clearance of malfunctioning mitochondria is an important housekeeping function in respiring eukaryotic cells, and plays a role physiological homeostasis as well the progression maternally inherited late‐onset diseases aging phenomena. Clearance entire mitochondrial comartments thought to occur using endo‐lysosomal system, by specific form autophagic degradation called mitophagy. In standard autophagy assays, such starvation‐induced macroautophagy, no mitophagy observed S. cerevisiae even at after very long periods time. We identified conditions where, absence external insult, massive occurs stationary phase cells. Our data suggest active for dynamics mediating mitophagy, opposed facilitating generating small, “eatable” fragments. This consistent with potential existence distillation process that utilizes fission fusion segregate defective components from functional components. To better understand intra‐mitochondrial segregation events during we carried out SILAC‐ based proteomic screen, searching proteins which behave differently mutants relative WT results allow us test formulate new hypotheses regarding protein sorting