Fos expression on medullary and hypothalamic areas after resistance exercise in rats.

• DOI: 10.1096/fasebj.27.1_supplement.lb730
• Publication Date: 2021-06-16T10:06:25Z
• Authors: Michael N Santana; Fabricio N Macedo; Vitor U Melo; Larissa R Oliveira; Robervan V dos Sa...; Waldecy L Junior; Marcio R Viana do...; Valter J de Santa...
• Keywords: 0303 health sciences; 03 medical and health sciences

The acute effects of exercise are generated by alterations in the autonomic nervous system that is mediated through the integration of multiple nuclei of autonomic control. The aim of this study was to evaluate the Fos protein expression on the hypothalamic and medullary areas of cardiovascular control after a single bout of resistance exercise (RE). Were used Wistar rats (250g–300g) divided into two groups: sham (SH) and resistance exercise (RE). The animals performed the RE protocol (10 sets of 10 repetitions with 2 min for recovery) in a squat apparatus with 60% of 1RM. The animals were anesthetized, perfused, the brain was removed and sliced in a cryostat for immunohistochemistry method. The brain sections were subjected to immunofluorescence protocol. Fos protein immunoreactivity increase when compared to SH in the nucleus tractus solitary (RE = 15.3 ± 0.6 vs. SH = 1.6 ± 0.6), in the caudal ventrolateral region of the medulla (RE = 23.3 ± 0.8 vs SH 1.3 ± 0.3) and in the paraventricular nucleus of the hypothalamus (RE = 59.3 ± 4.2 vs SH 1.3 ± 0.3), we showed that after RE there is an intense activation of important brain areas for cardiovascular control during the post exercise period. Suggesting mobilization and possible interaction of specifics nuclei of brain to correct homeostatic changes produced by RE. Research support: CAPES, Fapitec.