The activity and molecular interactions of extracellular EMMPRIN

• DOI: 10.1096/fasebj.27.1_supplement.lb82
• Publication Date: 2021-06-16T10:06:25Z
• Authors: Agnieszka A Kendrick; Elan Z Eisenmesser
• Keywords: Basigin

The Extracellular Matrix Metalloproteinase Inducer (EMMPRIN, CD147) is highly expressed in multiple cancers, particularly pancreatic cancer where it stimulates the secretion of entire classes proteins including matrix matelloproteinases (MMPs) and pro‐inflammatory cytokines. Although, EMMPRIN was initially identified as a cellular transmembrane glycoprotein, extracellular has recently emerged key player disease progression. described study here aims to directly characterize glycosylated regard its stimulatory activity target interactions, thereby unraveling critical mechanism that underlies We have developed recombinant expression systems purify both unglycosylated EMMPRIN. Our biological studies assessed difference between cells. Furthermore, we performed crosslinking pull‐down experiments, followed by mass spectrometry identify specific interacting partners. Thus, our determined functional influence glycosylation on EMMPRIN, begun interaction partners EZE supported NIH application number 1R01g‐01A1.