Cav1.1 controls ATP release to elicit gene expression in adult muscle fibers (1164.12)

• DOI: 10.1096/fasebj.28.1_supplement.1164.12
• Publication Date: 2021-06-16T09:49:38Z
• Authors: Gonzalo Jorquera; Denisse Valladares; France Piétri‐Rouxel; Bruno Allard; Vincent Jacquemond; Enrique Jaimovich; Mariana Casas
• Keywords: Pannexin; Adenosine triphosphate

In adult muscle fibers, Cav1.1 acts as voltage sensor for both excitation‐contraction coupling and the activation of a signaling cascade that regulates gene expression. We have shown ATP is released through pannexin‐1 channels after electrical stimulation at 20 Hz, having key role in induction transcriptional changes related to fast‐to‐slow fiber phenotype transition. Myotubes lacking Cav1.1α1 subunit displayed almost no release stimulation. transcription was also altered fibers knock‐down Cav1.1α1. dystrophic there an increase basal release, but default stimulation‐dependent observed control fibers. This can be interpreted uncoupling between muscles. All these data suggest controls trough Pannexin‐1 channels, activating it low frequencies blocking during resting conditions. The loss this perturbs normal response activity may play etiology muscular dystrophy. Grant Funding Source : ACT 1111, FONDECYT 1110467, UINICIA 0212M, CNRS‐CONICYT EDC24748