Dynamic shifts in transcription factor occupancy drive reprogramming of gene expression during hematopoietic differentiation (LB181)

• DOI: 10.1096/fasebj.28.1_supplement.lb181
• Publication Date: 2021-06-15T17:01:18Z
• Authors: Ross Hardison; Weisheng Wu; Christapher Morri...; Cheryl Keller; Tejaswini Mishra; Maxim Pimkin; Gerd Blobel; Mitchell Weiss; David Bodine
• Keywords: Chromatin immunoprecipitation; Reprogramming; ChIP-sequencing; GATA2; ChIA-PET

Global views of biochemical mechanisms regulating gene expression during cell lineage commitment and maturation can be obtained by analyzing chromatin immunoprecipitation on a genome-wide scale, enabled massively parallel sequencing. We have conducted ChIP-seq RNA-seq assays cells from many informative stages mouse hematopoiesis, ranging multilineage progenitor to mature megakaryocytes erythroblasts. find that histone modification patterns characteristic repressed or active are established commitment. The forms an accessible stage which transcription factors change their binding dynamically. Sites factors, such as GATA2/1 TAL1, both specific lineages terminal maturation. These shifts strongly associated with changes in expression, allowing proliferation early dramatic morphology function Motif searches coupled against reveal co-associating proteins help drive the dynamic key factors. Grant Funding Source: Supported NIH grants R01DK065806 RC2HG005573