Nucleosome Dynamics Around a DNA Double Stranded Break During Repair by Gene Conversion.
0301 basic medicine
0303 health sciences
03 medical and health sciences
DOI:
10.1096/fasebj.29.1_supplement.709.8
Publication Date:
2021-06-21T18:40:16Z
AUTHORS (3)
ABSTRACT
In eukaryotes, double‐stranded break repair (DSB) takes place in the context of chromatin. Saccharomyces cerevisiae mating type ( MAT ) switching provides an opportunity to investigate nucleosome dynamics around a well‐characterized DSB. is initiated by synchronous induction DSB HO endonuclease at locus following which ends are resected and Rad51 recombinase loaded on 3‐ended single strand. facilitates strand invasion with homologous donor sequence, required for initiation new DNA synthesis resulting repair. A hurdle studying positioning has been weak relative heterochromatic donors HML HMR . To address this we designed novel system inserted Xenopus laevis 5S sequence adjacent locus. Using have, first time, monitored loss chromatin protection resection end re‐establishment structure We show that lost resection‐dependent manner. Further, reestablished but not level seen prior This reestablishment depends passage through cell cycle. complete after several cycles, suggesting there mechanistic difference between repair‐dependent replication‐dependent positioning.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (0)
CITATIONS (1)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....