Sodium‐independent Dietary Effects on Renal Immune Cell Infiltration in Salt‐sensitive Hypertension

• DOI: 10.1096/fasebj.29.1_supplement.811.11
• Publication Date: 2021-06-21T17:03:12Z
• Authors: Justine Abais; Alexander Mattson; Brittany Wade; Hayley Lund; Nathan Rudemiller; Galina Petrova; David Mattson
• Keywords: 2. Zero hunger; 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; 3. Good health

The Dahl SS rat is a well-established model of human salt-sensitive hypertension and renal disease. Recently, sodium-independent effects diet, particularly during gestation, were shown to be important in the development this disease phenotype. SS/JrHsdMcwiCrl (SS/Crl) rats fed grain-based diet (Teklad 5L2F) less susceptible salt-induced blood pressure elevation damage compared SS/JrHsdMcwi (SS/Mcw) casein-based (AIN-76A). With known role immune system hypertension, present study characterized cells infiltrating kidney both SS/Mcw SS/Crl rats. Following 3 week high salt (4.0% NaCl) challenge, displayed significantly CD45+ leukocyte infiltration than (47.3% reduction, n=6-7). Specifically, there respective decreases CD3+ T cells, CD45R+ B CD11b/c+ antigen presenting by 52.4%, 77.7%, 45.9%, versus SS/Mcw. appears activated, as demonstrated 44.8% 66.8% reduction CD4+CD25+ CD8+CD25+ well 54.9% decrease CD4+CD25+FoxP3+ regulatory cells. also protected from damage, indicated attenuated urinary albumin (20±2 vs 99±16 mg/day) protein (73±4 173±32 excretion (n=6). These differences between show that dietary may influence response kidney, ultimately impacting susceptibility damage. Support: DK96859, HL116264, 5T32HL007792.