Increased Serotonin Availability Contributes to Decreased Bone Density in Colitis

• DOI: 10.1096/fasebj.29.1_supplement.854.5
• Publication Date: 2021-06-21T17:05:54Z
• Authors: Brigitte Lavoie; Jane Roberts; Stephanie Spohn; Keith Sharkey; Jane Lian; Gary Mawe
• Keywords: 0301 basic medicine; 03 medical and health sciences

The link between inflammatory bowel disease (IBD) and decreased bone density is not completely understood. It known that serotonin can have a negative impact on formation via preosteoblast 5-HT1B stimulation, serotonin-selective reuptake transporter (SERT) expression in inflamed intestinal epithelium, leading to increases 5-HT entering the blood stream from gut. Therefore, we tested hypothesis acting receptors contributing factor colitis-related deficits. Colitis was induced by adding dextran sodium sulfate (DSS) drinking water of BALB/c mice for 21 days (5% 7 then 1%). Some animals also received antagonist, GR55562 (1 mg/kg/day SC). DSS serum had higher levels compared control. Femurs were analyzed micro-computed tomography. treated exhibited marked decrease density, including significant deceases volume fraction, trabecular number surface, increased spacing. Treatment with antagonist improved all these features formation. Dynamic growth evaluated dual injections calcein 4 apart spacing fluorescent bands found be significantly reduced mice, but comparable control values antagonist. In conclusion, findings support novel concept altered handling gut factory colitis-induced changes density. Supported DK62267