AT1 Receptors Modulate Ca2+ Transient in Adult Cardiomyocytes Treated with T3

• DOI: 10.1096/fasebj.29.1_supplement.952.12
• Publication Date: 2021-06-21T16:57:21Z
• Authors: Ana Paula Taniguti; Eduardo Nocchi; Pedro Almeida; Silvia Guatimosim; Maria Luiza Barre...
• Keywords: 0301 basic medicine; 03 medical and health sciences

Increased thyroid hormone (TH) levels promote cardiac hypertrophy and arrhythmia by direct actions on myocardium interaction with other systems as local renin‐angiotensin system (RAS). Previous studies have confirmed that T3 rapidly increases AT1R angiotensin II protein in cultured cardiomyocytes. In the present study, we evaluated contribution of Ca 2+ transient cardiomyocytes treated T3. Adult ventricular (n=07) were isolated for 30 minutes (15nM, Sigma), plus losartan (LOS, 1µM, Sigma) or LOS alone. Intracellular (Ca i ) imaging experiments performed Fluo‐4 AM (10µM; Invitrogen) loaded‐cardiomyocytes electrically stimulated at 1 Hz.The confocal was a Zeiss LSM 510META microscope. The level reported F/F0, where F0 is resting Ca2+ fluorescence. treatment induced significant increase (3,53±0,88 vs. 2,89±0,69 Control, ANOVA, p蠄0.05) adult cardimyocytes, which attenuated concomitant (3,08±0,79 T3+LOS T3, p蠄0,05) demonstrating possible action inducing Supported FAPESP #2012/18515‐4