Renal Oxidation of ESS diabetic rats is minimized by EPA

• DOI: 10.1096/fasebj.29.1_supplement.967.1
• Publication Date: 2021-06-21T16:57:21Z
• Authors: Natalia Osiecki; Tomás Diaz; Adriana Ruiz Pecchio; Aldo Eynard; Gastón Repossi; Néstor García
• Keywords: 0301 basic medicine; 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; 3. Good health

Salt sensitivity (SS) is associated with increased cardiovascular risk in diabetic patients. This abnormal mechanism results an renal oxydation and decreased urinary sodium excretion if it maintained over time can develop hypertension its complications. Currently the Western diet has limited content of polyunsaturated fatty acids, antioxidant capacity such as ecoisapentaneoico acid (EPA). Therefore we hypothesized that nutritional supplementation EPA, prevents SS DM rats by decreasing oxidative stress. Our objectives were; to asses eSS investigate ability EPA prevent these rats. Methods Wistar were used healthy controls. Type II rat group (eSS), 3 months old, divided groups, control treated arachidonic (pro‐oxidant)(2.5mg / ip, per month) (eSS + AA) (2.5mg ip EPA). Animals during 1 year, then placed metabolic cages subsequently underwent 2 experimental periods 7 days each normal (0.4% NaCl)(NNaD) high salt (4% NaCl)(HNaD). At end period weight, systolic blood pressure (SBP), HbA1c, triglycerides (Trig), cholesterol (Chol), creatinine (Cre), kidney γ‐glutamyl transpeptidase activity (γGTP)​​, protein (U prot V). Results had elevated Tri, Chol reduced body weight vs. group. Renal function was period. The AA also showed elevation Trig, no change Cre Chol. During NNaD SBP 119 ± mmHg after HNaD 125 (p <0.05). In contrast, lowered HbA1c (5.6 0.3% vs 7.0 0.2%, p <0.05), Trig (175±1 mg/dl 245±12 (424±11 g 511±22gr, <0.05) eSS. supplement prevented increase (126±2 128±2 p> 0.5). did not differ from No significant difference observed U V between groups. Urinary HNAD. interstitium γ‐GT 0.65±0.04 au 0.61±0.02 group, while 0.5±0.03 Conclusion sentitive have hypertriglyceridemia HbA1c. Nutritional sensitivity, triglycerides. These changes lower ​​activity, suggesting dietary be patients improve abnormalities.