Trial‐to‐Trial and Day‐to‐Day Variability in Forearm Blood Flow During Reactive Hyperemia

• DOI: 10.1096/fasebj.29.1_supplement.lb611
• Publication Date: 2021-06-21T19:16:25Z
• Authors: Katrina D'Urzo; Meghan Plotnick; Troy Stuckless; Kyra Pyke
• Keywords: Reactive hyperemia; Brachial artery; Coefficient of variation; Cuff

Reactive hyperemia (RH) following the release of a brief limb occlusion is commonly used to assess resistance vessel function. The purpose this study was examine impact repeated trials on within subject day‐to‐day variability forearm blood flow during RH. Ten young, healthy, non‐smoking subjects (6 female, 4 male) were examined in fasted state. Brachial artery diameter and velocity assessed with Echo Doppler ultrasound, respectively. Subjects visited lab twice performed four RH each visit. protocol left arm included 5 minutes cuff at pressure 250 mmHg, followed by release. Within trial‐to‐trial coefficient variation (CV = (Standard Deviation/Mean) x 100). Day‐to‐day CV calculated using 1) Trial 1 from day 2) 4‐Trial average. Peak did not differ significantly between or days (p > 0.05). consistent visits (CV, 9.39 ± 7.78% 10.1 3.19%; p 0.737). determined first trial different average 14.9± 15.1% 12.0± 9.62%; 0.240). In conclusion, averaging over visit reduce peak reactive flow.