Optimization of the Hemodynamic of the Kidney and Preservation of Glycocalyx During Kidney Transplantations ‐ A Combination of Fluid Therapy and Low Dose Norepinephrine

• DOI: 10.1096/fasebj.30.1_supplement.950.10
• Publication Date: 2023-11-26T16:23:51Z
• Authors: Jonathan Kunisch ...; Niels Moeslund; Lise Have Nielsen; Jens Aage Kølsen ...; Bente Jespersen; Henrik Birn

Background Delayed Graft Function (DGF) is a common complication to kidney‐transplanted patients receiving kidney from brain dead donors. The present study designed evaluate the influence of fluid therapy on function and preservation glycocalyx, during transplantation using kidneys Method A porcine prospective randomized animal performed. recipients are either high volume or individualized goal directed (IGDT). pigs also no Nor‐epinephrine (NE) continuous low dose NE‐infusion. Brain death achieved by inflating 60cc urinary catheter inserted in epidural space until intracranial pressure exceeds systolic pressure. After 4 hours perfused with “Bridge life®” removed stored cooled at 4°C for least 18 hours. bilaterally nephrectomised each gets one kidney. recipient observed 10 post‐reperfusion. Blood urine samples collected this observation period. Biopsies taken donor baseline after 3 death, immediately reperfusion post reperfusion. will be evaluated measured GFR (mGFR) studying histology light microscopy. We plan glycocalyx degradation analyzing blood Syndecan‐1 Heparan Sufalte ELISA. Further histology, biopsies studied microscope, along immunofluorescence evaluation semi‐quantitative measurement glycocalyx. Results As an ongoing results have yet been obtained. Conclusion hope show significantly better IGDT group, even group NE. Support Funding Information This Study funded Novo Nordic fund.