Investigation of the Mechanism of Cytotoxic Natural Product PateamineA analog

• DOI: 10.1096/fasebj.31.1_supplement.lb114
• Publication Date: 2023-11-26T17:36:45Z
• Authors: Sai Shilpa Kommaraju; Woon‐Kai Low; Daniel Romo
• Keywords: Natural product; EIF4E; eIF4A; EIF4G; Mechanism of Action; Eukaryotic translation

Natural products have served as a source for many modern therapeutics. Over the past decade, high throughput screening of libraries small molecule compounds been popular in drug discovery. One such natural product is PateamineA (PatA). The PatA isolated from marine sponge Mycale hentscheli has found to be highly potent antiproliferative agent with potential novel anti‐neoplastic agent. and simplified analog desmethyl, desamino (DMDAPatA) cytotoxicity selective rapidly proliferating cells by inhibition cap‐dependent translation initiation through binding eIF4A (eukaryotic factor 4A) eIF4F complex. complex consists (RNA helicase), eIF4E (cap‐binding protein) eIF4G (Scaffolding protein). consequence impact other components on DMDAPatA action are investigated vitro cultured mammalian cells. Preliminary results demonstrate role cap‐binding mechanism action.