Patterns of gene expression in atrophying skeletal muscles: response to food deprivation

• DOI: 10.1096/fj.02-0312com
• Publication Date: 2002-10-30T18:44:20Z
• Authors: R. Thomas Jagoe; Stewart H. Lecker; Marcelo Gomes; Alfred L. Goldberg
• Keywords: Protein turnover; Protein Degradation; Muscle Atrophy

During fasting and many systemic diseases, muscle undergoes rapid loss of protein functional capacity. To define the transcriptional changes triggering atrophy energy conservation in fasting, we used cDNA microarrays to compare mRNAs from muscles control food-deprived mice. Expression >94% genes did not change, but interesting patterns emerged among that were differentially expressed: 1) encoding polyubiquitin, ubiquitin extension proteins, (but all) proteasome subunits increased, which presumably contributes accelerated breakdown; 2) a dramatic increase mRNA for ligase, atrogin-1, most E3s; 3) significant suppression myosin binding H other myofibrillar proteins) IGF 5, may favor cell loss; 4) decreases several glycolytic enzymes phosphorylase kinase subunits, increases pyruvate dehydrogenase 4 glutamine synthase, should promote glucose sparing gluconeogenesis. metallothionein increased dramatically, extracellular matrix components fell, cap-independent translation rose. Significant occurred growth-related proteins regulators. These indicate complex adaptive program degradation suppress oxidation muscle. Similar analysis atrophying causes is allowing us identify set atrophy-specific gene expression.