Bystander effect induced by counted high‐LET particles in confluent human fibroblasts: a mechanistic study

• DOI: 10.1096/fj.02-1115com
• Publication Date: 2003-07-30T17:02:14Z
• Authors: Chunlin Shao; Yoshiya Furusawa; Yasuhiko Kobayashi; Tomoo Funayama; Seiichi Wada
• Keywords: Bystander effect; Microbeam

The possible mechanism of a radiation-induced bystander response was investigated by using high-LET heavy particle microbeam, which allows selected cells to be individually hit with precise numbered particles. Even when only single cell within the confluent culture one 40Ar (approximately 1260 keV/microm) or 20Ne 380 keV/microm), 1.4-fold increase micronuclei (MN) detected demonstrating response. When number targeted increased, MN biphasically increased; however, efficiency induction per markedly decreased. 49 in were 1 4 particles, production irradiated cultures approximately 2-fold higher than control levels but independent and LET irradiated-culture partly reduced treatment DMSO, scavenger reactive oxygen species (ROS), almost fully suppressed mixture DMSO PMA, an inhibitor gap junctional intercellular communication (GJIC). Accordingly, both ROS GJIC contribute above-mentioned may play essential role mediating release soluble biochemical factors from cells.