Cellular prion protein promotes invasion and metastasis of gastric cancer

• DOI: 10.1096/fj.06-6138fje
• Publication Date: 2006-07-29T04:57:35Z
• Authors: Yanglin Pan; Lina Zhao; Jie Liang; Jie Liu; Yongquan Shi; Na Liu; Guoyun Zhang; Haifeng Jin; Juan Gao; Huahong Xie; Jun Wang; Zhiguo Liu; Daiming Fan; Yanglin Pan; Lina Zhao; Jie Liang; Jie Liu; Yongquan Shi; Na Liu; Guoyun Zhang; Haifeng Jin; Juan Gao; Huahong Xie; Jun Wang; Zhiguo Liu; Daiming Fan
• Keywords: Flavonoids; 0301 basic medicine; Prions; CD8-Positive T-Lymphocytes; 3. Good health; Mice; 03 medical and health sciences; Hyaluronan Receptors; Stomach Neoplasms; Calcium-Calmodulin-Dependent Protein Kinases; Cell Adhesion; Tumor Cells, Cultured; Animals; Humans; Neoplasm Invasiveness; Enzyme Inhibitors; Neoplasm Metastasis; Organic Chemicals

Cellular prion protein (PrPc) is a glycosylphosphatidylinositol (GPI) -anchored membrane that highly conserved in mammalian species. PrPc has the characteristics of adhesive molecules and thought to play role cell adhesion signaling. Here we investigated possible process invasiveness metastasis gastric cancers. was found be expressed metastatic cancers compared nonmetastatic ones by immunohistochemical staining. significantly promoted adhesive, invasive, vivo abilities cancer lines SGC7901 MKN45. also increased promoter activity expression MMP11 activating phosphorylated ErK1/2 cells. MEK inhibitor PD98059 antibody (Ab) inhibited vitro invasive induced PrPc. N-terminal fragment (amino acid 24-90) suggested an indispensable region for signal transduction invasion-promoting function Taken together, present work revealed novel existence could promote cells at least partially through activation MEK/ERK pathway consequent transactivation MMP11.