Docosahexaenoic acid (DHA) blunts liver injury by conversion to protective lipid mediators: protectin D1 and 17S‐hydroxy‐DHA

• DOI: 10.1096/fj.06-6250fje
• Publication Date: 2006-10-21T00:49:10Z
• Authors: Ana González‐Périz; Anna Planagumà; Karsten Gronert; Rosa Miquel; Marta López‐Parra; Esther Titos; Raquel Horrillo; Natàlia Ferré; Ramon Deulofeu; Vicente Arroyo; Juan Rodés; Joan Clària; Ana González‐Périz; Anna Planagumà; Karsten Gronert; Rosa Miquel; Marta López‐Parra; Esther Titos; Raquel Horrillo; Natàlia Ferré; Ramon Deulofeu; Vicente Arroyo; Juan Rodés; Joan Clària
• Keywords: Malondialdehyde

Docosahexaenoic acid (DHA) is a omega-3 essential fatty that reduces the incidence and severity of number diseases. Recently, novel series DHA-derived lipid mediators with potent protective actions has been identified. In this study we demonstrate dietary amplification these products protects liver from necroinflammatory injury. vitro, supplementation hepatocytes DHA significantly reduced hydrogen peroxide-induced DNA damage, evaluated by "comet assay," oxidative stress, determined measurement malondialdehyde levels. vivo, mice ameliorated carbon tetrachloride-induced damage. addition, hepatic cyclooxygenase-2 expression PGE2 levels were in fed DHA-enriched diets. animals, increased formation (i.e., 17S-hydroxy-DHA (17S-HDHA) protectin D1) was detected HPLC-gas chromatography/mass spectrometry analysis. Consistent findings, synthetic 17-HDHA abrogated genotoxic damage decreased TNF-alpha release 5-lipoxygenase macrophages. transactivation assay, acted concentration-dependent manner as PPARgamma agonist. Taken together, findings identify potential role for products, specifically 17S-HDHA D1, mediating effects