CD82 endocytosis and cholesterol‐dependent reorganization of tetraspanin webs and lipid rafts

• DOI: 10.1096/fj.08-123414
• Publication Date: 2009-06-05T00:46:57Z
• Authors: Congfeng Xu; Yanhui H. Zhang; Muthusamy Thangavel; Mekel M. Richardson; Li Liu; Bin Zhou; Yi Zheng; Rennolds S. Ostrom; Xin A. Zhang
• Keywords: Tetraspanin; Pinocytosis; Lipid raft; Bulk endocytosis; Internalization

Tetraspanin CD82 suppresses cell migration, tumor invasion, and metastasis. To determine the mechanism by which inhibits motility, most studies have focused on surface CD82, forms tetraspanin-enriched microdomains (TEMs) with other transmembrane proteins, such as integrins. In this study, we found that undergoes endocytosis traffics to endosomes lysosomes. endocytic of demonstrated dynamin clathrin are not essential for internalization. Depletion or sequestration sterol in plasma membrane markedly inhibited CD82. Despite demand Cdc42 activity, is distinct from macropinocytosis documented dynamin-independent pinocytosis. As a TEM component, reorganizes TEMs lipid rafts redistributing cholesterol into these microdomains. CD82-containing characterized cholesterol-containing extreme light- intermediate-density fractions. Moreover, appears alleviate CD82-mediated inhibition migration. Taken together, our demonstrate lipid-dependent drives trafficking late lysosomes, through redistribution cholesterol.