Bimgene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development

• DOI: 10.1096/fj.201700010r
• Publication Date: 2017-04-27T01:00:35Z
• Authors: Débora M. Cerqueira; Andrew J. Bodnar; Yu Leng Phua; Rachel Freer; Shelby L. Hemker; Loren D. Walensky; Neil A. Hukriede; Jacqueline Ho
• Keywords: Dicer; Progenitor

Low nephron endowment at birth has been associated with an increased risk for developing hypertension and chronic kidney disease. We demonstrated in earlier study that conditional deletion of the microRNA (miRNA)-processing enzyme Dicer from progenitors results premature depletion expression proapoptotic protein Bim (also known as Bcl-2L11). In this study, we generated a compound mouse model both Bim, to determine biologic significance Dicer-deficient progenitors. The loss partially restored number improved formation. undergoing apoptosis was significantly reduced kidneys single allele, or alleles, compared mutant kidneys. Furthermore, 2 miRNAs expressed (miR-17 miR-106b) regulated levels vitro vivo. Together, these data suggest miRNA-mediated regulation controls progenitor survival during nephrogenesis, one potential means regulating endowment.—Cerqueira, D. M., Bodnar, A. J., Phua, Y. L., Freer, R., Hemker, S. Walensky, L. D., Hukriede, N. A., Ho, J. gene dosage is critical modulating absence microRNAs development. FASEB 31, 3540–3554 (2017). www.fasebj.org