Altered cargo proteins of human plasma endothelial cell–derived exosomes in atherosclerotic cerebrovascular disease

• DOI: 10.1096/fj.201700149
• Publication Date: 2017-05-06T00:20:15Z
• Authors: Edward J. Goetzl; Janice B. Schwartz; Maja Mustapic; Iryna V. Lobach; Richard Daneman; Erin L. Abner; Gregory A. Jicha
• Keywords: Exosome; Endothelial Dysfunction

Plasma endothelial cell–derived exosomes (EDEs) and platelet-derived (PDEs) were precipitated enriched separately by immunospecific absorption procedures for analyses of cargo proteins relevant to atherosclerosis. EDEs had usual exosome size marker protein content, significantly higher levels than PDEs the vascular cell adhesion molecule-1 (VCAM-1) nitric oxide synthase, whereas platelet glycoprotein VI. EDE VCAM-1, von Willebrand factor, growth factor (PDGF)-BB, angiopoietin-1, lysyl oxidase-2 cerebrovascular-selective glucose transporter 1, permeability-glycoprotein, large neutral amino acid 1 18 patients with cerebrovascular disease (CeVD) age- gender-matched control subjects. PDE PDGF-AA, VI, integrin-linked kinase-1, high mobility group box-1 protein, chemokine CXCL4, thrombospondin-1 in CeVD subjects, but differences less greater overlaps proteins. Yes-associated (YAP) P(S127)-YAP lower consistent heightened activity this mechanical force–sensitive system Elevated atherosclerosis-promoting justify clinical studies their potential value as biomarkers.—Goetzl, E. J., Schwartz, J. B., Mustapic, M., Lobach, I. V., Daneman, R., Abner, L., Jicha, G. A. Altered human plasma atherosclerotic disease. FASEB 31, 3689–3694 (2017). www.fasebj.org