Analysis of chromatin accessibility in decidualizing human endometrial stromal cells

• DOI: 10.1096/fj.201701098r
• Publication Date: 2017-12-19T15:50:54Z
• Authors: Pavle Vrljicak; Emma S. Lucas; Lauren Lansdowne; Raffaella Lucciola; Joanne Muter; Nigel P. Dyer; Jan J. Brosens; Sascha Ott
• Keywords: Decidualization; Decidua

Spontaneous decidualization of the endometrium in response to progesterone signaling is confined menstruating species, including humans and other higher primates. During this process, endometrial stromal cells (EnSCs) differentiate into specialized decidual that control embryo implantation. We subjected undifferentiated decidualizing human EnSCs an assay for transposase accessible chromatin with sequencing (ATAC-seq) map underlying changes. A total 185,084 open DNA loci were mapped accurately EnSCs. Altered accessibility upon was strongly associated differential gene expression. Analysis 1533 opening closing regions revealed over-representation binding motifs known transcription factors (TFs) identified putative new regulators. ATAC-seq footprint analysis provided evidence TF at specific motifs. One largest footprints involved most enriched motif-basic leucine zipper-as part a triple motif also comprised estrogen receptor Pax domain sites. Without exception, located within Alu elements, which suggests role primate-specific transposable element (TE) evolution genes. Although TEs generally under-represented EnSCs, several classes contributed regulatory landscape underpins expression.-Vrljicak, P., Lucas, E. S., Lansdowne, L., Lucciola, R., Muter, J., Dyer, N. Brosens, J. Ott, S. cells.