MicroRNA‐351‐5p mediates skeletal myogenesis by directly targeting lactamase‐β and is regulated bylnc‐mg

• DOI: 10.1096/fj.201701394rrr
• Publication Date: 2018-09-14T18:04:52Z
• Authors: Jingjing Du; Peiwen Zhang; Xue Zhao; Jin He; Yan Xu; Qin Zou; Jia Luo; Linyuan Shen; Hao Gu; Qianzi Tang; Mingzhou Li; Yanzhi Jiang; Guoqing Tang; Lin Bai; Xuewei Li; Jinyong Wang; Shunhua Zhang; Li Zhu
• Keywords: Ribosomal Proteins; 0301 basic medicine; Myoblasts, Skeletal; Membrane Proteins; Muscle Proteins; Cell Differentiation; Muscle Development; Cell Line; Mice; MicroRNAs; 03 medical and health sciences; Muscle Fibers, Slow-Twitch; Animals; RNA, Long Noncoding; Cell Proliferation

Skeletal muscle is an important and complex organ with a variety of functions in humans and animals. Skeletal myogenesis is a multistep and complex process, and increasing evidence suggests that microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) play critical roles in skeletal myogenesis. In this study the expression of miR-351-5p is dynamically regulated during skeletal myogenesis in vitro and in vivo. Cell-counting kit-8, qRT-PCR, and EdU immunofluorescence analysis showed that miR-351-5p overexpression promoted the proliferation and inhibited the differentiation of C2C12 myoblast, whereas inhibition of miR-351-5p had the opposite effect. In addition, miR-351-5p mediated the regulation of muscle fiber type transition in vivo. In vitro, loss of miR-351-5p in muscle tissues promoted muscle hypertrophy and increased slow-twitch fibers in the gastrocnemius muscles of mice. Luciferase reporter assay and functional analyses demonstrated that lactamase β ( LACTB) is a direct target of miR-351-5p involved in the regulation of skeletal myogenesis. Expression levels of a myogenesis-associated lncRNA ( lnc-mg) correlated negatively with miR-351-5p and positively with LACTB during C2C12 myoblast proliferation and differentiation. Further analyses showed that lnc-mg acted as a molecular sponge for miR-351-5p, demonstrating its involvement in the negative regulation of LACTB by miR-351-5p during skeletal myogenesis. These findings indicate that miRNA-351-5p functions in skeletal myogenesis by targeting LACTB and is regulated by lnc-mg, supporting the role of the competing endogenous RNA network in skeletal myogenesis.-Du, J., Zhang, P., Zhao, X., He, J., Xu, Y., Zou, Q., Luo, J., Shen, L., Gu, H., Tang, Q., Li, M., Jiang, Y., Tang, G., Bai, L., Li, X., Wang, J., Zhang, S., Zhu, L. MicroRNA-351-5p mediates skeletal myogenesis by directly targeting lactamase β and is regulated by lnc-mg.