Ablation of the microRNA‐17‐92 cluster in neural stem cells diminishes adult hippocampal neurogenesis and cognitive function

• DOI: 10.1096/fj.201801019r
• Publication Date: 2019-01-22T20:57:09Z
• Authors: Wan Long Pan; Michael Chopp; Baoyan Fan; Ruilan Zhang; Xinli Wang; Jiani Hu; Xiao Ming Zhang; Zheng Gang Zhang; Xian Shuang Liu
• Keywords: Neuroblast

Impairment of adult neurogenesis in the hippocampus causes cognitive deficits; however, underlying molecular mechanisms have not been fully elucidated. microRNAs (miRNAs) regulate neural stem cell (NSC) function. With use a transgenic mouse line with conditional ablation miR-17-92 cluster nestin lineage NSCs, we tested hypothesis that regulates and function vivo. Compared wild-type mice, significantly reduced number proliferating NSCs neuroblasts neuronal differentiation dentate gyrus (DG) impaired hippocampal-dependent learning memory, as assayed by social recognition novel object recognition, Morris water-maze tests. Statistical analysis showed highly significant correlation between newly generated DG cognition deficits knockout (KO) mice. Western blot KO increased cytoskeleton-associated protein, Enigma homolog 1 (ENH1), its downstream transcription factor, inhibitor (ID1), respectively, well phosphatase tensin gene. These proteins are related to differentiation. Our study demonstrates is critical for behavioral may target ENH1/ID1 signaling.—Pan, W. L., Chopp, M., Fan, B., Zhang, R., Wang, X., Hu, J., X. Z. G., Liu, S. Ablation microRNA-17-92 cells diminishes hippocampal FASEB J. 33, 5257–5267 (2019). www.fasebj.org