Distinct expression pattern of periostin splice variants in chondrocytes and ligament progenitor cells

• DOI: 10.1096/fj.201802281r
• Publication Date: 2019-04-17T00:31:18Z
• Authors: Lei Cai; Robert H. Brophy; Eric D. Tycksen; Xin Duan; Ryan M. Nunley; Muhammad Farooq Rai
• Keywords: Periostin; Progenitor; splice

Periostin (POSTN), a secretory matricellular matrix protein, plays multitude of biologic functions. Various splice variants POSTN have been described; however, their expression pattern and functional implications are not completely understood. This study was undertaken to decipher the differential its in various tissues cell types. We show that more highly expressed anterior cruciate ligament (ACL) remnants compared with articular cartilage at cellular tissue level. Isoforms 1 8 were only chondrocytes, suggesting splice-specific regulation chondrocytes. To discern role total full-length human isoform (hPOSTN-001), we stably transfected chondrosarcoma (hCh-1) line hPOSTN-001 using pcDNA3.1–hPOSTN-001 construct. RNA-sequencing analysis hCh-1 cells identified differentially genes known chondrocyte function osteoarthritis. Similar subset candidate revealed ACL progenitor chondrocytes as well which activity altered by overexpression small interfering RNA gene knockdown. Cells expressing POSTN, 1, exhibited increased adhesion potential. These findings suggest an important for knee.—Cai, L., Brophy, R. H., Tycksen, E. D., Duan, X., Nunley, M., Rai, M. F. Distinct periostin cells. FASEB J. 33, 8386–8405 (2019). www.fasebj.org