Downregulated ribosomal protein L39 inhibits trophoblast cell migration and invasion by targeting E‐cadherin in the placenta of patients with preeclampsia

• DOI: 10.1096/fj.202002061r
• Publication Date: 2021-03-12T14:51:21Z
• Authors: Qiuling Jie; Fei Sun; Qi Li; Juan Zhu; Yunjian Wei; Huamei Yang; Ping Long; Zhen Wang; Xiaohui Yang; Dan Li; Liping Huang; Yanlin Ma
• Keywords: Trophoblast

Abstract Early‐onset preeclampsia (PE) is a pregnancy complication that can lead to severe adverse maternal and fetal outcomes. However, the mechanisms underlying development of early‐onset PE are not fully understood. Ribosomal protein L39 (RPL39) member S39E family ribosomal proteins plays an important role in stem cell self‐renewal, cancer metastasis, chemoresistance. In this study, we aimed explore potential function RPL39 placental trophoblast cells. We analyzed expression normal tissues using real‐time PCR, western blot analysis, immunohistochemistry. The results showed was markedly downregulated tissues. knockdown inhibited proliferation, migration, invasion, as well explant outgrowth. Flow cytometry analysis suggested resulted cycle arrest at G0/G1 phase, but had no significant effect on apoptosis. also found could alter morphology. then measured epithelial marker E‐cadherin following Bewo HTR8/SVneo increased E‐cadherin. Furthermore, upregulated outgrowth transfected with small interfering RNA. conclusion, essential migration cells by targeting Our findings provide novel insight into occurrence PE.