Single nucleus and spatial transcriptomic profiling of healthy human hamstring tendon

• DOI: 10.1096/fj.202300601rrr
• Publication Date: 2024-05-14T12:05:58Z
• Authors: Jolet Y. Mimpen; Lorenzo Ramos‐Mucci; Claudia Paul; Alina Kurjan; Philippa A. Hulley; Chinemerem T. Ikw...; Carla J. Cohen; Stephen E. Gwilym; Mathew J. Baldwin; Adam P. Cribbs; Sarah J. B. Snelling
• Keywords: Cell type

Abstract The molecular and cellular basis of health in human tendons remains poorly understood. Among tendons, hamstring tendon has markedly low pathology can provide a prototypic healthy reference. aim this study was to determine the transcriptomes location all cell types tendon. Using single nucleus RNA sequencing, we profiled 10 533 nuclei from four donors identified 12 distinct types. We confirmed presence two fibroblast types, endothelial cells, mural immune previously unreported including different skeletal muscle satellite adipocytes, undefined nervous system cells. these within defined using spatial transcriptomics imaging, potential transcriptional networks cell–cell interactions were analyzed. demonstrate that fibroblasts have highest number our dataset, are present throughout tendon, play an important role production organization extracellular matrix, thus confirming their as key regulators homeostasis. Overall, findings underscore complexity underpin function central tissue