Activation of moderate autophagy promotes survival of fat graft

• DOI: 10.1096/fj.202300892r
• Publication Date: 2023-11-11T11:34:35Z
• Authors: Su‐Jun Pei; Yuan‐Zheng Zhu; Juan‐Min Yang; Min‐Chen Zhang; Chen‐Long Shi; Ying Ding; Yang‐Yan Yi
• Keywords: Sirolimus; Mice; Ischemia; Cell Survival; Graft Survival; Autophagy; Animals; Mice, Nude; Reactive Oxygen Species; 3. Good health

Clinically unpredictable retention following fat grafting remains outstanding problems because of the unrevealed mechanism grafted survival. The role autophagy, a process to maintain cellular homeostasis through recycling debris, has yet been be reported in grafting. This study aims improve survival autophagy. First, relationship between cell death and autophagy early stage was evaluated immunostaining, RNA sequencing, western blot. Next, rapamycin, an autophagic agonist, used for culturing adipose-derived stem cells adipocytes during ischemia. Cell death, reactive oxygen species (ROS) were assayed. Finally, rapamycin assist nude mice. results demonstrated that peak at accompanied by decrease In vitro, ischemia, 25 nM confirmed as optimal dose reduces with enhanced mitophagy, improved mitochondrial quality well decreased ROS accumulation. vivo, promoted alleviated oxidative stress, apoptosis rapamycin-treated grafts observed stage. addition, increased neovascularization reduced fibrosis. We suggested moderate induced contribute ischemic tolerance long term control.