Wolfberry, Yam, and Chrysanthemum polysaccharides increased intestinal Akkermansia muciniphila abundance and hepatic YAP1 expression to alleviate DILI

• DOI: 10.1096/fj.202301388r
• Publication Date: 2023-11-11T11:26:37Z
• Authors: Junlan Lu; Yi Gong; Yuting Gao; Yanguang Yang; Yuman Zhang; Zhiqin Zhang; Xinli Shi
• Keywords: Akkermansia muciniphila; Lycium

Drug-induced liver injury (DILI) is frequently induced by high dose of acetaminophen (APAP) and concomitant with disturbances gut flora. Akkermansia muciniphila beneficial for the repair injury. Lycium barbarum polysaccharide, yam chrysanthemum polysaccharide all have anti-inflammatory antioxidation effects. The objective this study to investigate potential lycium (LYC) in improving DILI increasing abundance A. muciniphila. Initially, screening optimal concentrations wolfberry, yam, (WYC) or LYC promote proliferation vitro validated antibiotic (ATB)-treated KM mice. Subsequently, APAP-induced model BALB/c mice were constructed examine treatment effects LYC. Our findings indicate that concentration ratio WYC was 2:3:2, 1:1:1. increased ATB-treated under ratio. Meanwhile, combination promoted overdose APAP resulted impairment intestinal barrier function subsequent leakage lipopolysaccharide (LPS). Moreover, abundance, reduced inflammation permeability, upregulated expression tight junction protein zonula occludens 1 (ZO-1) occludin contents gut. Lastly, inhibited LPS hepatic YAP1 expression, ultimately leading DILI.