Oxidized phospholipid POVPC contributes to vascular calcification by triggering ferroptosis of vascular smooth muscle cells

0301 basic medicine Cyclohexylamines Phosphorylcholine Myocytes, Smooth Muscle Phenylenediamines Muscle, Smooth, Vascular 03 medical and health sciences Osteogenesis Humans Ferroptosis Reactive Oxygen Species Vascular Calcification Phospholipids Cells, Cultured
DOI: 10.1096/fj.202302570r Publication Date: 2024-04-06T08:55:28Z
ABSTRACT
Abstract Vascular calcification is an actively regulated biological process resembling bone formation, and osteogenic differentiation of vascular smooth muscle cells (VSMCs) plays a crucial role in this process. 1‐Palmitoyl‐2‐(5′‐oxo‐valeroyl)‐sn‐glycero‐3‐phosphocholine (POVPC), oxidized phospholipid, found atherosclerotic plaques has been shown to induce oxidative stress. However, the effects POVPC on VSMCs have yet be studied. In present study, we investigated using vitro ex vivo models. increased mineralization arterial rings, as by alizarin red staining. addition, treatment expression markers Runx2 BMP2, indicating that promotes transition VSMCs. Moreover, stress impaired mitochondria function VSMCs, ROS levels, impairment mitochondrial membrane potential, decreased ATP levels. Notably, ferroptosis triggered was confirmed levels intracellular ROS, lipid MDA, which were ferrostatin‐1, inhibitor. Furthermore, ferrostatin‐1 attenuated POVPC‐induced Taken together, our study for first time demonstrates via activation VSMC ferroptosis. Reducing or inhibiting might provide novel strategy treat calcification.
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