Stromal‐Like Cells and Retinal Pigment Epithelium Modulate Choroidal Sprouting Through Galectin‐1‐Dependent and Independent Pathways

• DOI: 10.1096/fj.202403181r
• Publication Date: 2025-05-23T15:00:02Z
• Authors: Magali E. Ridano; Christin Hanke‐Go...; Guillermo L. Lehmann; Paulo S. Caceres; Maria V. Vaglienti; Danilo Ceschin; Ryan Schreiner; Diego O. Croci; Carlos Torroja‐Fu...; Enrique Rodriguez...; Ignacio Benedicto; Gabriel A. Rabino...; María C. Sánchez
• Keywords: Choroid

ABSTRACT Outer retinal function depends on two supporting tissues: the pigment epithelium (RPE) and choroid. Limited molecular information is available intercellular networks that sustain RPE/choroid tissue in both healthy pathological states. Galectin‐1 (Gal1), a β‐galactoside‐binding lectin, has recently emerged as key regulator of angiogenesis potential therapeutic target vascular pathologies, including age‐related macular degeneration. Here, we studied expression Gal1 outer retina its regulatory role under physiological conditions. Our findings indicate predominantly associated with stromal cells RPE/choroid. In Gal1‐deficient ( Lgals1 −/− ) mice, ultrastructure gene profiles were altered, choroidal explants exhibited reduced sprouting compared to those wild‐type mice. Consistently, recombinant promoted hypoxic conditions, stromal‐like modulated pro‐angiogenic antiangiogenic vitro Interestingly, was also expressed by RPE, apical secretion normoxia shifted toward basolateral phenotype hypoxia. These identify RPE sources choroid, highlighting distinct roles maintaining homeostasis or microenvironments.