XENOGRAFT REJECTION OF PORCINE ISLET-LIKE CELL CLUSTERS IN IMMUNOGLOBULIN- OR Fc-RECEPTOR ??-DEFICIENT MICE1

• DOI: 10.1097/00007890-199611150-00003
• Publication Date: 2004-09-03T08:13:28Z
• Authors: Birgitta Benda; Alex Karlsson-Parra; Anna Ridderstad; Olle Korsgren
• Keywords: Fc receptor; Fragment crystallizable region

The aim of the present study was to evaluate role xenoreactive antibodies in islet-like cell cluster (ICC) xenograft rejection. For this purpose, normal mice, mice with a targeted disruption Fc-receptor (FcR) γ-chain, or membrane exon immunoglobulinμ-chain gene, were transplanted fetal porcine ICC under kidney capsule. Mice lacking FcR γ have no functional for IgG IgE. immunoglobulin μ-chain cannot produce antibodies, because B development is arrested at stage preB cells. All animals, irrespective recipient group, readily rejected xenograft. Analyses pattern cellular infiltration revealed only minor dissimilarities between different experimental groups. Xenograft destruction evident on day 6 after transplantation, and large number mononuclear cells found be evenly distributed throughout graft. majority infiltrating large, macrophage-like expressing macrophage-specific phenotype marker F4/80. CD3-positive T lymphocytes mainly accumulated peripheral parts This has demonstrated that are not crucial rejection pig-to-mouse model.