NUMBER AND REPLATING CAPACITY OF ENDOTHELIAL COLONY FORMING CELLS ARE TELOMERE LENGTH DEPENDENT: IMPLICATION FOR HUMAN ATHEROGENESIS

• DOI: 10.1097/01.hjh.0000747008.62647.7f
• Publication Date: 2021-04-09T03:38:33Z
• Authors: Simon Toupance; Stéphanie Simoncini; Carlos Labat; Chloé Dumoulin; Tsung-Po Lai; Oualid Ayad; Cécile Lakomy; Abraham Aviv; Françoise Dignat-...; Florence Sabatier; Athanase Benetos
• Keywords: Clonogenic assay; clone (Java method)

Abstract Objective: Short leukocyte telomere length (TL) is associated with atherosclerotic cardiovascular disease. Endothelial repair plays a key role in the development of atherosclerosis. To explore potential short TL atherogenesis, we examined associations between and proliferative dynamics endothelial colony forming cells (ECFCs), which behave as progenitor displaying activity. Design method: isolate ECFCs, performed clonogenic assay on blood samples from 116 participants (24 to 94 years old) TELARTA (Telomere Arterial Aging) study. We detected no ECFC clone 29 (Group 1), clones replating capacity other 2), additional 58 3). was measured by Southern blotting leukocytes (LTL) ECFCs (ECFC-TL). Results: Age- sex-adjusted LTL (mean ± SEM) shortest Group 1 (6.51 0.13 kb), longer 2 (6.69 kb) longest 3 (6.78 0.09 (p < 0.05). In group 3, ECFC-TL number 0.01). (7.98 than (6.74 0.012 0.0001) both parameters were strongly correlated (r = 0.82; p 0.0001). Conclusions: Individuals telomeres display higher self-renewing suggesting that they might have better capacity. Our results also indicate LTL, proxy could be used surrogate marker clinical laboratory practice due easy accessibility leukocytes.