PD12-09 DEVELOPMENT OF MALE AND FEMALE MODELS OF LONG URETHRAL STRICTURES IN SWINE

• DOI: 10.1097/01.ju.0001008772.30001.48.09
• Publication Date: 2024-04-15T21:33:57Z
• Authors: Gokhan Gundogdu; Babak Azad; Travis Nguyen; Ekaterina Perminov; David Barham; Joel Gelman; Joshua R. Mauney

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology (PD12)1 May 2024PD12-09 DEVELOPMENT OF MALE AND FEMALE MODELS LONG URETHRAL STRICTURES IN SWINE Gokhan Gundogdu, Babak Azad, Travis Nguyen, Ekaterina Perminov, David Barham, Joel Gelman, and Joshua R. Mauney GundogduGokhan Gundogdu , AzadBabak Azad NguyenTravis Nguyen PerminovEkaterina Perminov BarhamDavid Barham GelmanJoel Gelman MauneyJoshua View All Author Informationhttps://doi.org/10.1097/01.JU.0001008772.30001.48.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION OBJECTIVE: Urethral stricture disease is a significant public health issue which occurs due scarring in or around the urethra that restricts blocks urine flow. Long urethral strictures (>2 cm length) routinely require one- two-stage urethroplasty with autologous tissues biomaterial grafts restore organ caliber mitigate urinary tract obstruction. Preclinical evaluations prospective graft configurations for are performed male rabbit models small scale, traumatic defects. These systems fail recreate fibrotic microenvironment dimensions long do not address gender-specific differences pathology. The objectives this study were develop both female porcine (∼4 characterize histological functional outcomes iatrogenic formation between genders. METHODS: Focal, partial thickness injuries created over 5-6 segments swine (N=4 per gender) via electrocoagulation degree was monitored up 6 weeks by urethroscopy retrograde urethrography. Animals sacrificed following confirmation histological, immunohistochemical, histomorphometric analyses on strictured uninjured control profile wound healing responses. RESULTS: animals survived period. detected all 2 100% at 3.2±1.8 weeks, post-operatively. mean length 4 cm. Substantial variations severity sexes observed males exhibiting stenosis loss α-smooth muscle actin+smooth bundles comparison controls, while females primarily displayed defects pan-cytokeratin+ epithelia as well CONCLUSIONS: Electrocoagulation injury sufficient produce nature obstruction be dependent gender. Source Funding: National Institutes Health grants, R01DK119240 (Mauney) R01DK131211 © 2024 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e259 Advertisement Copyright Permissions© Inc.Metrics Information More articles author Expand PDF downloadLoading ...