MP28-14 NOVEL IN VIVO PEYRONIE'S PATIENT-DERIVED XENOGRAFT MODEL RECAPITULATES HUMAN CURVATURE IN IMMUNOCOMPROMISED RATS

• DOI: 10.1097/01.ju.0001008872.42208.7a.14
• Publication Date: 2024-04-15T21:33:57Z
• Authors: Garrett Brinkley; Jacob W. Greenberg; Danish Singh; Asim B. Abdel-Mageed; Wayne J. G. Hells...
• Keywords: Tunica albuginea (penis)

You have accessJournal of UrologySexual Function/Dysfunction: Peyronie's Disease (MP28)1 May 2024MP28-14 NOVEL IN VIVO PEYRONIE'S PATIENT-DERIVED XENOGRAFT MODEL RECAPITULATES HUMAN CURVATURE IMMUNOCOMPROMISED RATS Garrett Brinkley, Jacob W. Greenberg, Danish Singh, Asim B. Abdel-Mageed, and Wayne J. G. Hellstrom BrinkleyGarrett Brinkley , GreenbergJacob Greenberg SinghDanish Singh Abdel-MageedAsim Abdel-Mageed HellstromWayne View All Author Informationhttps://doi.org/10.1097/01.JU.0001008872.42208.7a.14AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: disease (PD) is a psychologically debilitating physically limiting condition with∼4% prevalence in the adult male population. Currently, there are few nonsurgical options, with paucity linked limited pool validated animal models available for preclinical research. Available do not mirror human PD tissue microenvironment origin but rather simulate TGF-β plaque pathophysiology. To address this issue, first time, study aimed form translational, preclinical, patient-derived xenograft (PDX) model mimicking pathogenesis that can be employed as predictive drug development. METHODS: In compliance institutional-approved protocols appropriate consent, fresh plaques were excised from patients subsequently implanted into tunica albuginea two immunocompromised (nude) rats. Rodents followed 3 months. Curvature was then assessed by using saline injected through 28-gauge needle lateral side corpora. measured midline proximal distal aspect point maximal curvature. Penile harvested, specimen fixed formalin, sectioned, stained H&E. RESULTS: A total rats PDX, only one PDX survived Artificial erection normal demonstrated 32° curvature (Figure 1a). The sent histologic evaluation. H&E staining persistent on dorsolateral penial shaft adjacent dorsal vein nerve bundle. further identified in-line Buck's facia, invading 1b). CONCLUSIONS: challenging treat standard care, including collagenase injections, plication, incision grafting, penile prosthesis placement. There an imminent need validate therapies, optimize current strategies, provide new treatment options. Management will progress without models. This pilot (that recapitulates vivo microenvironment) validates feasibility developing Additional optimization analysis warrants investigation future studies. Download PPT Source Funding: No funding © 2024 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e477 Advertisement Copyright & Permissions© Inc.Metrics Information More articles author Expand PDF downloadLoading ...