Correlation of p16INK4A Overexpression with Human Papillomavirus Infection in Cervical Adenocarcinomas

• DOI: 10.1097/01.pgp.0000092135.88121.1f
• Publication Date: 2003-11-18T13:08:37Z
• Authors: Mitsuya Ishikawa; Takuma Fujii; Nobuo Masumoto; Miyuki Saito; Makio Mukai; Ingo Nindl; Ruediger Ridder; Takeshi Fukuchi; Kaneyuki Kubushiro; Katsumi Tsukazaki; Shiro Nozawa
• Keywords: Immunostaining; clone (Java method)

As human papillomavirus (HPV) infection is the main risk factor for squamous cell carcinoma of cervix and overexpression p16INK4a occurs when retinoblastoma protein inactivated by high-risk HPV, authors studied association HPV expression in cervical adenocarcinomas. Specimens glandular neoplasias were immunostained with a p16INK4a-specific monoclonal antibody (clone E6H4). Approximately 80% neoplasms showed p16INK4a. Exfoliated cells from 14 adenocarcinomas further examined immunocytochemistry, 12 cases p16INK4a, suggesting that immunostaining may be useful diagnostic tool The DNA polymerase chain reaction method. Overexpression was positive 94% which HPV16 or 18DNA positive, finding 18 play an important role indicator pathogenic activity HPVs.