P.127: The Influence of Temperature Conditions and Reconstruction of the Extracellular Matrix Environment on the Viability of Pancreatic Islets

• DOI: 10.1097/01.tp.0000804580.93058.ad
• Publication Date: 2021-11-28T00:00:20Z
• Authors: Michał Wszoła; Marta Klak; Grzegorz Tymicki; Magdalena Gomółka; Patrycja Kowalska; Tomasz Bryniarski; Andrzej Berman
• Keywords: Decellularization; Pancreatic Islets; Islet cell transplantation

Introduction: Pancreatic islet transplantation is one of the methods treating patients with T1D. However, for procedure to be successful, high quality implanted islets key importance a successful transplant. Following isolation, it has been shown that mainly β-cells undergo apoptosis due vascular loss and hypoxia. The cellular stresses which are subjected during isolation (for example disturbance cell-matrix relationship). Appropriate storage conditions insulated islands their functionality. most common method introduction into suspension culture. because these micro organs, pancreatic more accurate term. Therefore, this study aimed demonstrate whether better cultivate them in standard (37oC; 5% CO2), or maybe stored at 4oC. Material: research material was porcine islets. Islets were isolated from native organ using collagenase Ricordi chamber. After divided 4 groups: 1. control (islands suspended CMRL 1066 medium) - traditional breeding (temperature 37oC; CO2); 2. 4oC; 3. medium addition dECM (decellularized extracellular matrix) culture conditions; 4. supplemented Each variant tested 3 replicates each trial 3000iEq. Additionally, on cleaned not immediately after isolation. Results: showed temperature, reduced 4oC, ECM significantly influenced functionality This (uncleaned islands) even 5 days On other hand, incubated 37°C without no 2 experiment. remaining variants gradual 4oC case. Conclusion: islets, as organs vascularization, need appropriate ensure proper functioning. Based studies, should cultivated single-cell lines. In addition, allows recreation matrix-cell environment, corresponding environment. financially supported by National Centre Research Development STRATEGMED3/305813/2/NCBR/2017.