Low-tidal-volume Mechanical Ventilation Induces a Toll-like Receptor 4–dependent Inflammatory Response in Healthy Mice

• DOI: 10.1097/aln.0b013e318182aef1
• Publication Date: 2008-08-15T07:11:16Z
• Authors: Michiel Vaneker; Leo A. Joosten; Leo M. A. Heunks; Dirk G. Snijdelaar; Feico J. Halbertsma; Jan van Egmond; Mihai G. Netea; Johannes G. van d...; Gert Jan Scheffer
• Keywords: Proinflammatory cytokine

Mechanical ventilation (MV) can induce ventilator-induced lung injury. A role for proinflammatory pathways has been proposed. The current studies analyzed the roles of Toll-like receptor (TLR) 4 and TLR2 involvement in inflammatory response after MV healthy lung.Wild-type (WT) C57BL6, TLR4 knockout (KO), KO mice were mechanically ventilated h. Bronchoalveolar lavage fluid was presence endogenous ligands. Lung homogenates used to investigate changes expression. Cytokines measured homogenate plasma, leukocytes counted tissue.MV significantly increased ligands bronchoalveolar relative messenger RNA expression tissue. In homogenates, WT levels keratinocyte-derived chemokine, interleukin (IL)-1alpha, IL-1beta. mice, IL-1alpha but not IL-1beta, increase chemokine less pronounced. IL-6, tumor necrosis factor alpha. did IL-6 or alpha, result different cytokine compared with mice. number pulmonary leukocytes.The study supports a reaction short-term lungs. Increasing understanding innate immune may lead future treatment advances injury, which serve as therapeutic target.