Delayed Treatment with Isoflurane Attenuates Lipopolysaccharide and Interferon γ–induced Activation and Injury of Mouse Microglial Cells

• DOI: 10.1097/aln.0b013e3181af5b3d
• Publication Date: 2009-08-19T07:13:15Z
• Authors: Jie-Ae Kim; Liaoliao Li; Zhiyi Zuo
• Keywords: Viability assay

Background Isoflurane pretreatment can induce protection against lipopolysaccharide and interferon gamma (IFNgamma)-induced injury activation of mouse microglial cells. This study's goal was to determine whether delayed isoflurane treatment is protective. Methods Mouse cells were exposed various concentrations for 1 h immediately after the initiation (10 or 1000 ng/ml) IFNgamma U/ml) stimulation 2% at times stimulation. Nitrite production, lactate dehydrogenase release, cell viability measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay assessed with 24 h. Inducible nitric oxide synthase (iNOS) protein expression quantified Western blotting. The iNOS in brain also studied. Results applied 0 2 improved viability. 2%, but not 3%, reduced IFNgamma-induced nitrite production decreased Aminoguanidine, an inhibitor, attenuated this Chelerythrine bisindolylmalemide IX, kinase C inhibitors, abolished effects on application. lipopolysaccharide-induced brain. Late application release that inhibited aminoguanidine. Conclusions These results suggest reduce may be mediated C.